Biochem/physiol Actions

Kd ~800 nM in binding to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins)

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetPromotes the catalytic activity of caspase-9 in the presence of XIAP

General description

A peptide that contains the N-terminal seven residues of Smac (Second mitochondria-derived activator of caspases, also known as DIABLO) and promotes pro-caspase-3 activation at ~10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) (K_d = ~800 nM). Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) with a K_d ~800 nM. Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Srinivasula, S.M., et al. 2001. Nature410, 112.Chai, J., et al. 2000. Nature406, 855.Liu, Z., et al. 2000. Nature408, 1004.Srinivasula, S.M., et al. 2000. J. Biol. Chem.275, 36152.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sequence

H-Ala-Val-Pro-Ile-Ala-Gln-Lys-OH

Warning

Toxicity: Standard Handling (A)